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更新时间:2022-11-10
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鍥? GLUT1缁撴瀯锛堝浘婧愶細[2]锛?/p> 聽 鏃㈡槸鐝犵帀鍦ㄥ墠锛屼负浣曟椂闂存柇妗ｏ紵 濡傛灉棰滃畞2014骞撮噰鐢ㄧ殑绉戠爺鏂规硶鍙互璁よ瘉闃庢鼎娑涙彁鍑虹殑鍋囪锛岄偅涔堥瀹佺殑鏂囩珷搴旇鎻愬埌闂崥澹?993鐨勮鏂囥€備絾鏄紝浠庡唴瀹规潵鐪嬶紝棰滃畞2014骞寸殑鏂囩珷娌℃湁寮曠敤1993骞撮槑娑︽稕鐨勬垚鏋溿€傞€氳繃鍒嗘瀽寮曠敤1993骞存枃绔犵殑鐮旂┒棰嗗煙鏉ョ湅锛屽苟鏃犳秹鍙奨鍏夋櫠浣撹灏勬柟娉曟柟闈紝浜︽垨璁告湁鐮旂┒鍋氫簡锛屼絾鏈垚鍔熴€傚湪1993骞磋嚦2014骞撮棿锛屾棤浜洪噰鐢ㄩ瀹佷娇鐢ㄧ殑鏂规硶鐮旂┒鍚屾牱鐨勯澏鐐癸紙浜烘簮钁¤悇绯栬浆杩愯泲鐧斤級锛岃繖璇存槑鍒汉娌℃兂鍒拌繖涓柟娉曘€備换浣曚汉濡傛灉鐪嬩簡1993骞寸殑鏂囩珷锛屽氨鑳借В鍑?014骞存枃绔犵殑鏅朵綋缁撴瀯锛岄偅鍦?993骞磋嚦2014骞撮棿灏卞簲璇ユ湁浜哄仛鍑烘潵浜嗭紝鍙幇瀹炲苟娌℃湁銆?/p> 聽聽 鐮旂┒棰嗗煙涓嶅悓锛岄毦瀹氣€滄妱琚€濅箣璇?/strong> 2014骞存枃绔犳妱琚?993骞存枃绔犵殑涓昏浜夋墽鐐瑰湪浜?993骞存枃绔犵殑鍥?鍜?014骞存枃绔犵殑鍥?銆?993鏂囩珷灞曠ず鐨勫崐鑳辨皑閰?65锛屽彲浠ュ湪涓嶅悓闃舵鎺ヨЕ鍒扮粏鑳炶啘鍐呭鐨勭幆澧冦€?014鏂囩珷閲岄潰鍥句竴鏄泲鐧借川鐨勪笁缁寸粨鏋勶紝灞曠ずinward-open conformation锛堝悜鍐呮墦寮€鐨勬瀯鍨嬶級锛涘浘浜旀樉绀哄彟涓€涓猻uperfamily 鐨勮泲鐧借川 D-xylose:H+ symporter 锛圶ylE锛?鐨勬櫠浣撶粨鏋勫凡瑙ｆ瀯锛屾槸outward open锛堝悜澶栨墦寮€锛夌殑鏋勫瀷锛屼笖閫氱瘒娌℃湁鎻愬埌鍗婅儽姘ㄩ吀鎴栬€呰泲鐧借川涓庡簳鐗?鎶戝埗鍓傜殑鐢熷寲鍙嶅簲銆?993骞寸殑鏂囩珷涓苟鏃?014骞村浘5鎵€绀虹殑姗欒壊鍚堥〉閮ㄥ垎锛屼袱绡囩爺绌舵秹鍙婄殑棰嗗煙骞朵笉鐩稿悓銆?/p>
鍥? 鏈夋妱琚珜鐤戠殑鐮旂┒鍐呭瀵规瘮锛堝浘婧愶細[1][2]锛?/p> 聽 棰滃畞鐮旂┒涔嬪墠锛屽凡鏈夆€滄秹瀚屸€濇枃绔?/strong> 棰滃畞浜?012骞碞ature鍙戣〃鏂囩珷鐨勬枃鐚?8鐨勭‘鍑虹幇浜哋utward facing partially occluded conformation涓€璇嶏紝鍗冲湪2012骞翠竴涓猳utward facing铔嬬櫧璐ㄦ瀯鍨嬬殑瀹炰緥宸茬粡鏈変簡锛屽姞涓婇瀹?014骞存枃绔犳妸鍙︿竴涓猧nward facing铔嬬櫧璐ㄦ瀯鍨嬩篃瑙ｆ瀽浜嗭紝閭ｄ箞G-6-P鑲畾璺ㄨ繃鑶滆繖涓凡鐭ヤ簨瀹炲緱鍒颁簡浜嬪疄璁よ瘉銆?/p> 2014骞撮瀹佺殑鏂囩珷鎵胯鏂囩尞28鍜屾枃鐚?9鐨勫彂鐜帮紝缁撳悎鑷繁鍙戠幇鐨勬櫠浣撶粨鏋勶紝缁樺埗浜嗗浘浜旓紝骞舵寚鍑篢MD璺烮CH鐩镐簰浣滅敤锛屾帶鍒跺悎椤电殑寮€鍏炽€傚浘浜旀槸涓ょ鏋勫瀷杞崲鐨勭ず鎰忓浘銆?strong>娌℃湁搴曠墿缁撳悎鐨勬椂鍊欙紝铔嬬櫧璐ㄥ洜涓哄悎椤佃窡TMD鐨勭浉浜掑惛寮曪紝铔嬬櫧璐ㄩ噰鐢╫utward-open鏋勫瀷锛涘簳鐗╄窡铔嬬櫧璐ㄥ湪primary site缁撳悎鍚庯紝璁╄泲鐧借川鐨勭晶閰哥鍜屾皑鍩虹闈犳嫝锛屽皝闂紑鍚戠粏鑳炶啘澶栫殑閫氳矾锛屽彉鎴恑nward-open 鏋勫瀷銆?/strong>绉戝鐣屾棭灏辩煡閬揋-6-P鑲畾浼氳法鑶滐紝浣嗗畠濡備綍绌胯繃鑶滅殑锛熷湪棰滃畞2014骞存枃绔犲彂琛ㄤ箣鍓嶆病浜虹煡閬撱€?/p>
鍥? 2012骞撮瀹佸湪Nature鍙戣〃鏂囩珷鐨勫弬鑰冩枃鐚?8锛堝浘婧愶細[3]锛?/p> 聽 1993骞存枃绔犵殑浣滆€呭簲璇ュ厛鍛婇瀹佸湪2012骞碞ature鍙戣〃鏂囩珷鐨勫弬鑰冩枃鐚?8娑夊珜鈥滄妱琚€濇墠鏇翠负鍏厑锛岀劧鍚庡啀鍛婇瀹?014骞村彂琛ㄧ殑鏂囩珷銆傚鏋滃彂琛ㄤ簬2012骞寸殑鏂囩尞28鍜屾枃鐚?9鐨勪綔鑰呭潎娌℃湁璁や负棰滃畞2014骞寸殑鏂囩珷鎶勮浜嗚嚜宸憋紝閭ｄ箞1993鐨勬枃绔犱綔鑰呬綍璋撻瀹佹妱琚紵 鎾版枃|鐭宠埆濉斿奖 鎺掔増|涔旂淮閽?/p> 鍙傝€冭祫鏂欙細 [1]Yan RT, Maloney PC. Identification of a residue in the translocation pathway of a membrane carrier.Cell. 1993 Oct 8;75(1):37-44. PMID: 8402899. [2]Deng D, Xu C, Sun P, et al. Crystal structure of the human glucose transporter GLUT1. Nature. 2014 Jun 5;510(7503):121-5. doi: 10.1038/nature13306. Epub 2014 May 18. PMID: 24847886. [3]Sun L, Zeng X, Yan C, et al. Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Nature. 2012 Oct 18;490(7420):361-6. doi: 10.1038/nature11524. PMID: 23075985. |
